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Miniscope GRIN Lens System for Calcium Imaging of Neuronal …
This article includes miniscope design, the surgical procedure for GRIN lens implantation, miniscope mounting on the head of a mouse, and data acquisition and analysis.
- Author: Lifeng Zhang, Bo Liang, Giovanni Barbera, Sarah Hawes, Sarah Hawes, Yan Zhang, Kyle Stump, Ira Baum,...
- Publish Year: 2019
Unit Title: Miniscope GRIN lens system for calcium imaging of …
Oct 18, 2019 · Here we present our custom-designed miniscope GRadient INdex (GRIN) lens system that enables simultaneously recording from hundreds of neurons for months. This …
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Surgery Protocol - UCLA Miniscope
See more on miniscope.orgBefore implanting the GRIN lens, you will need to inject a fluorescent indicator such as GCaMP6. We have generally used AAV1.Syn.GCaMP6f.WPRE.SV40 from Penn Vector (Item number AV-1-PV2822) and had great success in dorsal CA1. You may also be able to use GCaMP6 transgenic mice, but we have not tested w…Stereotaxic Viral Injection and Gradient-Index Lens Implantation …
The preparation procedure consists of three steps, including (1) stereotaxically injecting the virus at the desired brain region of a mouse brain to label a specific subgroup of neurons with …
A Two-Step GRIN Lens Coating for In Vivo Brain Imaging - PMC
We developed a GRIN lens-coating method that allows the use of any commercial GRIN lens for in vivo neuroscience research with excellent imaging quality. This method rivals the output …
Clear optically matched panoramic access channel …
Aug 5, 2021 · Such miniature lens probes typically utilize a GRIN lens for its slim cylindrical body to form a focus deep inside brain tissue and relay the optical …
- Author: Bowen Wei, Chenmao Wang, Zongyue Cheng, Zongyue Cheng, Baoling Lai, Wen-Biao Gan, Meng Cui
- Publish Year: 2021
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Gradient Index Microlens Implanted in Prefrontal Cortex of Mouse …
Micro-optic implants, such as GRIN lenses, are well suited for combined mode, longitudinal imaging studies in mouse brain regions that are too deep to reach through standard …
Stereotaxic Viral Injection and Gradient-Index Lens ... - PubMed
Oct 8, 2021 · The preparation procedure consists of three steps, including (1) stereotaxically injecting the virus at the desired brain region of a mouse brain to label a specific subgroup of …
Aberration correction in long GRIN lens-based microendoscopes …
Oct 21, 2024 · In this study, we combined optical simulations with fabrication of aspherical polymer microlenses through three-dimensional (3D) microprinting to correct for optical …
Calcium imaging of adult-born neurons in freely moving mice
Mar 3, 2021 · Surgically implant a gradient-index (GRIN) lens and electroencephalogram (EEG)/electromyogram (EMG) electrodes into the brains of tamoxifen-injected mice. Note: We …
Two-photon imaging in freely behaving mice - Nature Methods
May 11, 2022 · The MINI2P can image through a cover glass, a gradient index (GRIN) lens or a prism, which the researchers illustrated by imaging in the visual cortex, the hippocampus or …
Light-guided sectioning for precise - Cell Press
Sep 28, 2021 · In GRIN-lens-implanted mice, we show how LiGS can add information to single-cell-resolution imaging. After two-photon GCaMP imaging, images acquired in vivo were registered to LiGS-processed tissue images in …
Time course images of cellular injury and recovery in murine …
Here, we present the design and use of an implanted small-diameter, high-resolution gradient index (hrGRIN) lens system for longitudinal studies that is optimized to image changes in fine …
A Two-Step GRIN Lens Coating for In Vivo Brain Imaging
Mar 9, 2019 · Here, we describe a protocol for GRIN lens coating that ensures successful long-term intravital imaging with commercially-available GRIN lenses. The complex spatial and …
Optical development in the zebrafish eye lens - Wang - 2020
Feb 26, 2020 · Results in the present study show that the GRIN changes with lens growth, particularly the proportions of the central plateau region to the total lens size and the …
Minimally invasive microendoscopy system for in vivo functional …
We designed a thin-walled polyimide guide cannula that minimizes tissue removal during its implantation in mouse brain, and allows easy insertion and removal of 0.5-mm-diameter GRIN …
Negative and positive auto-regulation of BMP expression in
Nov 15, 2015 · Our results show that chicken embryo pre-lens ectoderm cells express BMPs and require BMP signaling for lens specification in vitro, and that in vivo inhibition of BMP signals in …
Role of Aquaporin 0 in lens biomechanics - PubMed
Jul 10, 2015 · Our recent ex vivo study on AQP0 knockout (AQP0 KO) mouse lenses showed the CTCA function of AQP0 could be crucial for establishing the refractive index gradient. …
Simultaneous Microendoscopic Calcium Imaging and EEG …
Here, we describe technical details and protocols for in vivo calcium imaging in the brainstem of sleeping mice. In this system, sleep-related neuronal activity in the ventrolateral medulla …
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